As far as I could figure out, retinal contains a reactive aldehyde group optimally converted to a stable product for accurate quantification. O-ethylhydroxylamine can be used used to convert retinal into a stable O-ethyl oxime derivative.
Is there any other way to measure retinal with HPLC without this step?
Kind regards
we have measured retinal and a series of eccentrically cleaved apo-carotenals directly by LCMS/MS. we have tried this with and without first deactivating reactive amines in samples with formaldehyde and found no difference suggesting that losses of retinal and other aldehydes is not a big problem. in addition, some of my colleagues have expressed concern with using derivatization methods to oxime in that it might artificially drive some reactions to create more aldehydes than are actually there.
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