For fixation of cells in 10% formalin with PBS . 1xPBS with 37% formaldehyde can be used
The buffer capacity for pH setting depend of PBS concentration. In your case only refer 1X (is not concentration unit). I dont have experience in this area but the PBS concentration should be checked. I think that use PBS 50 - 100 mM this solution can be neutral using formalin. Regards
To adjust the 10% formalin solution to a neutral pH you would need to mix in quantities of a buffer, typically sodium phosphate. A recommended recipe is as follows:
100ml Formalin (37-40% stock solution)
900ml Water
4 g/L NaH2PO4 (monobasic)
6.5 g/L Na2HPO4 (dibasic/anhydrous)
10% formalin can also be referred to as formal or formol. A common example is formal saline which is another method of fixative preparation using sodium chloride and sodium phosphate to buffer as follows:
100ml Formalin (37-40% stock solution)
900ml Water
9 g NaCl
12 g Na2HPO4 (dibasic/anhydrous
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