Can ITS1F - ITS4 primer pair can be employed instead of ITS1-ITS4 pair for identification of yeasts?

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Giorgia Pertile

These two primer is more use to determine the fungal biomass.

Rumakanta Sapkota

Dear Bhaskar,

Yes, you can actually use any one of them. ITS1F is better than ITS1 if you are working in the plant DNA background. But if you wanted to identify yeast species from pure cultures, you can use any one of them.

Bhaskar Jyoti Nath

Thank you for your valuable answers. I will proceed as suggested. Thank you.