Bhaskar Jyoti Nath

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Questions related from Bhaskar Jyoti Nath

Can ITS1F - ITS4 primer pair can be employed instead of ITS1-ITS4 pair for identification of yeasts?

AS far as I know, both ITS1F and ITS1 amplify the ITS1-5.8s-ITS2 region, but from literature so far, I found ITS1 primer is being particularly used for yeasts where as ITS1F is referred specific...

03 March 2018 3,556 3 View

What is a suitable culture media for ethanol production test and a reliable biochemical method to estimate the produced ethanol?

We are working on yeast isolates and are interested to screen high ethanol producing isolates. Since the number of isolates is quite high ( >200), it is not possible for us to analyze the whole...

11 November 2014 5,993 6 View

We accidentaly procurred two enzymes Pronase and Lyticase from Sigma but unfortunately we dont need them now..anybody interested to buy from us??

In April 2014 we ordered two enzymes Pronase and Lyticase (Zymolase 20T) from Merck and sigma to conduct an experiment for estimating mannan expression in yeasts..however we had to discontinue the...

07 July 2014 10,042 3 View

Is it normal to get OD at 600 of about 15-17 after 24 hours incubation for yeast isolates?

While preparing a growth curve for certain yeast isolates, after 24 hours I got the OD reading ranging from 15 to 17 (sometimes 21), where as most literature suggest OD 600 of maximum 1 after 24...

01 January 2014 8,932 11 View

Is there any standard chart for OD Vs CFU for determining a microbial population?

I need to standardize the growth phases of yeast cells as quick as possible. However, with the plate count and haemocytometer method due to the large no. of isolates. So, can anyone provide me...

01 January 2014 8,478 8 View