We accidentaly procurred two enzymes Pronase and Lyticase from Sigma but unfortunately we dont need them now..anybody interested to buy from us??

More Bhaskar Jyoti Nath's questions See All

Can ITS1F - ITS4 primer pair can be employed instead of ITS1-ITS4 pair for identification of yeasts?

AS far as I know, both ITS1F and ITS1 amplify the ITS1-5.8s-ITS2 region, but from literature so far, I found ITS1 primer is being particularly used for yeasts where as ITS1F is referred specific...

02 March 2018 3,506 3 View

What is a suitable culture media for ethanol production test and a reliable biochemical method to estimate the produced ethanol?

We are working on yeast isolates and are interested to screen high ethanol producing isolates. Since the number of isolates is quite high ( >200), it is not possible for us to analyze the whole...

10 November 2014 5,904 6 View

Is it normal to get OD at 600 of about 15-17 after 24 hours incubation for yeast isolates?

While preparing a growth curve for certain yeast isolates, after 24 hours I got the OD reading ranging from 15 to 17 (sometimes 21), where as most literature suggest OD 600 of maximum 1 after 24...

31 December 2013 8,886 11 View

Is there any standard chart for OD Vs CFU for determining a microbial population?

I need to standardize the growth phases of yeast cells as quick as possible. However, with the plate count and haemocytometer method due to the large no. of isolates. So, can anyone provide me...

31 December 2013 8,381 8 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View