Can IL6 upregulate TNF-a and IL-8 in in vitro cell culture model?

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Every cytokine has its own signaling pathway and don't effect other cytokines expression?

Is it correct to say that IL-6 has a seperate signaling pathway and don't effect expression of TNF -a in cell culture model ? Any answer appriciated.

02 March 2019 5,876 41 View

What is the best way to calculate the ELISA result?

I have used excel and Prism to analyse the results. I have some sample values which are below blank (OD). If I substract the OD value of these samples from blank I do get VERY LOW or negative...

01 February 2019 614 7 View

A co-cultured stimulated IL-6 (An inflammatory model) does not show the upregulation of proinflammatory cytokines. Is the model working fine?

I am working on a in vitro inflammatory model(a co-cultured model) which stimulated with IL-6. After stimulation ,the control and control+IL-6 show no significatnt differences in expersion of...

01 February 2019 520 5 View

What is the best assay to confirm the result from ELISA?

Hi, I am checking the antiinflammatory effect of few substances on a co-culture model. I have screened the pro-inflammatory cytokines with ELISA kits. Do I need to confirm the result with...

31 December 2018 9,645 8 View

Can ELISA on cell lysate give more detectable results for cytokines compare to basolateral medium?

Can ELISA on cell lysate give better and detectable results for cytokines compare to basolateral medium?

31 December 2018 6,976 3 View

Does phenol red interfer with ELISA?

Hi, I am going to run an ELISA- a colorimetric based method. I have phenol red in my samples. I read both negative and nutral impact of phenol red on ELISA. I want to know if really phenol red...

11 December 2018 6,505 3 View

What are the best method to asssess the establishment of integrity in invitro intestinal monolayer?

I am working with Caco-2 cells. I seed them on transwell filter. In our group TEER has been used to assess the the monolayer integrity. However TEER has been unreliable due to high flactuation. We...

09 October 2018 9,441 9 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View