Every cytokine has its own signaling pathway and don't effect other cytokines expression?

Is it correct to say that IL-6 has a seperate signaling pathway and don't effect expression of TNF -a in cell culture model ? Any answer appriciated.

02 March 2019 5,764 41 View

What is the best way to calculate the ELISA result?

I have used excel and Prism to analyse the results. I have some sample values which are below blank (OD). If I substract the OD value of these samples from blank I do get VERY LOW or negative...

01 February 2019 438 7 View

A co-cultured stimulated IL-6 (An inflammatory model) does not show the upregulation of proinflammatory cytokines. Is the model working fine?

I am working on a in vitro inflammatory model(a co-cultured model) which stimulated with IL-6. After stimulation ,the control and control+IL-6 show no significatnt differences in expersion of...

01 February 2019 405 5 View

Can ELISA on cell lysate give more detectable results for cytokines compare to basolateral medium?

Can ELISA on cell lysate give better and detectable results for cytokines compare to basolateral medium?

31 December 2018 6,872 3 View

What is the best assay to confirm the result from ELISA?

Hi, I am checking the antiinflammatory effect of few substances on a co-culture model. I have screened the pro-inflammatory cytokines with ELISA kits. Do I need to confirm the result with...

31 December 2018 9,550 8 View

Does phenol red interfer with ELISA?

Hi, I am going to run an ELISA- a colorimetric based method. I have phenol red in my samples. I read both negative and nutral impact of phenol red on ELISA. I want to know if really phenol red...

11 December 2018 6,332 3 View

What are the best method to asssess the establishment of integrity in invitro intestinal monolayer?

I am working with Caco-2 cells. I seed them on transwell filter. In our group TEER has been used to assess the the monolayer integrity. However TEER has been unreliable due to high flactuation. We...

09 October 2018 9,308 9 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View