Does phenol red interfer with ELISA?

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Can IL6 upregulate TNF-a and IL-8 in in vitro cell culture model?

Hi, I am working with an inflammatory cell culture model which the stimulater is IL6 10 ng /ml . But after checking the pro-inflammatory cytokines like TNF-a ,IL8, INF-a , non of them has been...

02 March 2019 4,342 5 View

Every cytokine has its own signaling pathway and don't effect other cytokines expression?

Is it correct to say that IL-6 has a seperate signaling pathway and don't effect expression of TNF -a in cell culture model ? Any answer appriciated.

02 March 2019 5,876 41 View

What is the best way to calculate the ELISA result?

I have used excel and Prism to analyse the results. I have some sample values which are below blank (OD). If I substract the OD value of these samples from blank I do get VERY LOW or negative...

01 February 2019 614 7 View

A co-cultured stimulated IL-6 (An inflammatory model) does not show the upregulation of proinflammatory cytokines. Is the model working fine?

I am working on a in vitro inflammatory model(a co-cultured model) which stimulated with IL-6. After stimulation ,the control and control+IL-6 show no significatnt differences in expersion of...

01 February 2019 520 5 View

What is the best assay to confirm the result from ELISA?

Hi, I am checking the antiinflammatory effect of few substances on a co-culture model. I have screened the pro-inflammatory cytokines with ELISA kits. Do I need to confirm the result with...

31 December 2018 9,645 8 View

Can ELISA on cell lysate give more detectable results for cytokines compare to basolateral medium?

Can ELISA on cell lysate give better and detectable results for cytokines compare to basolateral medium?

31 December 2018 6,976 3 View

What are the best method to asssess the establishment of integrity in invitro intestinal monolayer?

I am working with Caco-2 cells. I seed them on transwell filter. In our group TEER has been used to assess the the monolayer integrity. However TEER has been unreliable due to high flactuation. We...

09 October 2018 9,441 9 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View

VECM Model: Positive/insignificant CointEq1 ?

Hello everyone, I am conducting

22 July 2024 8,085 11 View

Merge Intensity Data from 2 runs?

Hi, We have collected several images for two independent runs of an experiment using two different confocal microscopes and settings. Thus, the intensity values collected through ImageJ were...

22 July 2024 8,722 0 View