Is it possible that first waiting time decreases and then after a certain point waiting time start increasing?

The sevice rate is fixed and the arrival rate is changing. As I am plotting the graph of arrival rate with respect to waiting time in queue.

22 December 2020 2,455 1 View

Is comparing genetic aberrations from NGS and Transcriptome data feasible?

I am doing an analysis with transcriptome data and comparing the same with the Genomic data. More precisely, I am looking at mRNA results of a tumor sample without having the normal tumor samples...

26 October 2020 3,793 3 View

How to compare two samples with different sample size?

I need to compare two samples, one sample has 500 data points and other sample has 100 data points? Which would be the best way to compare them? I learned online that T-test will not be the best...

26 April 2020 2,869 4 View

How to reduce the simulation running time in sentaurus tcad as it takes 7-8 hour.. to complete the simulation..?

I have simulated the device, but it takes much time to complete the simulation..

02 January 2020 3,683 2 View

How to apply Chi square test in insect Bioassay ?

We have insect mortality number as well as mortality percentage , but while considering chi square test which should calculate either number of insect died or its mortality percentage..kindly do...

15 December 2019 3,061 4 View

What are the different methods to study microbial populations in soil?

I want to know about the different methods to study microbial populations in soil. Microbial population means taxonomy, what type of microorganisms are present in soil up to genus level. Kindly...

22 July 2019 1,601 2 View

How to calculate Young's Modulus of plastic sheets using Load vs Extension plot and Stress vs Strain plot?

I have the plots for Load vs Extension as well as Stress vs Strain for different kinds of plastic sheets. I used the standard ASTM D638 for preparation of specimen and conducting the experiment as...

24 June 2019 7,964 3 View

Can we take the average of critical difference (CD) value of the 2 year data on which statistics was performed on each year's data individually?

I have two year data and performed statistics on each year data individually. Now I got the mean and critical difference (CD) value of each year's data but I want to represent the data as mean...

01 February 2019 3,816 2 View

How to explain the increase in the near-infrared emission intensity upon heating the tta based Ln complexes?

In my experiment, firstly i measured NIR luminescence for the complex at 5 K, 100 K, and 300 K without heating which revealed very weak luminescence intensity. However, upon heating the sample to...

02 January 2019 931 1 View

What is property of water and hair when there is a thin layer of water?

I observed that when the surface is wet i.e, there is a very thin layer of water on the surface and if one hair thread falls on it, then the region near by hair get dry. That is water is being...

22 September 2018 7,553 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View