In high demand CPD courses in basic laboratory techniques?

Hi, We are seeking to establish CPD courses covering basic laboratory techniques for technicians, graduate students and other interested parties. I am thus seeking advice on the sort of courses...

27 February 2018 3,019 5 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

What's the right way to Tween 20 storage?

02 December 2020 9,955 2 View

Why my IP injcetion drug for mice turn opaque after thawing?

Hi, I have prepared a drug solution that is dissolved in DMSO. We are giving IP injection to mice. The final stock concentration is 2mg/ml in 5% DMSO, 2% Tween-80 and 93% Saline. Before injection,...

18 November 2020 6,000 2 View

How to break TPP-chitosan NPs? How to avoid aggreation?

Hey, hi, I am preparing Chitosan-TPP NPs in the ratio 1 (TPP) : 5 (CHI), I am encapsulating DNA inside the NPs and to measure the E.E. I am spinning down the particles and measuring the...

26 October 2020 9,898 3 View

Can anybody suggest way to take healthy food for long life?

Due to the epidemic that urge millions to get strong immunity.now we all seek good ,cheap and available food for long healthy life.some researchers suggest these food. tables and fruits that have...

27 September 2020 2,713 11 View

How to prepare nanoemulsion at home without a ultrasonic probe sonicator ?

I am trying to make a nano-emulsion to convert a cancer medicine into its more bioavailable form following guidance from this patent https://patents.google.com/patent/US9259391B2/en But I made...

17 September 2020 9,192 1 View

How much tween 20 or ethanol should i use in order to dissolve 1 ml of essential oil?

Is there any standard or procedure for this? Thanks in advance.

07 September 2020 5,053 3 View

Help with Direct ELISA?

Hi, I am trying to do a direct ELISA for mouse IGF-1 (only standards for now), however I am facing technical issues with obtaining a lower blank value and a linear graph. I am doing the following...

03 August 2020 6,260 11 View

I did a plant extraction using ethanol and dried to make a powder. Now i want to dissolve the powder again in water. How can i do that?

I have used both tween 80 and 20 before dry the plant extract that may increase solubility. However, that couldn't make the dried product soluble in water.

22 July 2020 6,435 13 View

What is the correct way to store my ELISA buffers?

Hello I was wondering what the correct storage for the following buffers was and how long they last: Coating buffer: Na2CO3-NaHCO3 pH9.6 Wash Buffer: PBS & 0.05% Tween 20 Antibody...

19 November 2019 4,030 3 View