Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

Reliable PGP9.5 antibody to visualize intraepidermal nerve fibres in rat paw skin?

Hi, I'm looking for a reliable PGP9.5 antibody that can visualize INEF in rat paw skin. Most publications with good image on rat paw uses Ultraclone or AbD Serotec's rabbit anti-human antibody...

19 February 2020 979 2 View

Are there protocols to extract steroid hormones and RNA from the same tissue?

Hi all, is it possible to extract RNA and steroid hormones from the same tissue? My current strategy is to macerate the animal tissue sample and divide into equal portions, one for TRIzol RNA...

28 July 2019 1,579 3 View

Which channel/filter to use on BioRad CFX96 for Thermal Shift Assay?

Thermal shift assay is very new to me so please bear with me. This is a very specific question and it does seem like a lot of people use BioRad CFX96 to do their Thermal Shift Assay using SYPRO...

15 December 2016 3,990 14 View

Any advice to remove background from western blots?

After performing western blot (oxyblot), I do see my bands, but I also get patchy background. and I notice it is usually the spots where I put my ECL on first then gets the background. I have no...

11 April 2016 7,229 3 View

Is there any commercial anti-EGFRvIII antibody for flow cytometry recommendation?

I want to test the EGFRvIII expression in my cell line. I am wondering if there is any commercial anti-EGFRvIII antibody for flow cytometry? Has anyone have this experience?

20 January 2016 1,183 1 View

Why do I get no protein expression in E coli?

Hi all, I have recently tried to transform a plasmid encoding a membrane protein-of-interest to E.coli C41(DE3). The plasmid is kanamycin resistant. It was transformed successfully but when I...

27 December 2015 174 6 View

How can I supplement 17alpha-methyltestosterone into L-15 cell culture?

15 December 2015 7,045 1 View

Can someone help me with in vitro functional analysis of exhausted CD8+ T cells?

22 February 2015 8,336 4 View

Is the order of impedance modulus value different with the corrosion resistance?

In the EIS tests, Zsimpwin software is taken to fit the results. My results show that the order of impadance modulus value is different from the oder of corrosion resistance (charge transfer...

02 July 2014 8,440 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View