Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View

Is rotary evaporator a must before going through TPC, TFC and DPPH assay?

I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...

23 October 2020 3,268 10 View

Can LentiCrisprV2 is used to knockout mouse gene?

Has anyone tried using addgene LentiCrisprV2 (Plasmid # 52961) to knockout a mouse gene? The cells were resistant to puro after infection, however, none of my gRNAs can knockout the gene.

19 May 2020 1,180 3 View

How to design concrete with different fly ash/binder ratios but the same strength level for cost comparison?

I want to design some concrete mixes with different fly ash/binder ratios (can be as high as 80%) but the same strength level. The water/binder ratios (together with superplasticizer dosages) have...

12 May 2020 8,551 6 View

Bugs in mouse genotyping?

Hi, I am genotyping newly constructed trangenic mice knocking down target gene. I have got F1 generation from F0. And DNA examination indicates that the target DNA fragment has been excised. The...

27 April 2020 5,450 3 View

In western blot, what kind of problem will cause antibodies stain the top area of each sample strip?

According to the color or the loading buffer and the protein ladder, the pull-down progressed successfully. And I also get right signals of beta-actin (lower ones), but another primary antibody...

28 August 2019 3,702 7 View

Are there protocols to extract steroid hormones and RNA from the same tissue?

Hi all, is it possible to extract RNA and steroid hormones from the same tissue? My current strategy is to macerate the animal tissue sample and divide into equal portions, one for TRIzol RNA...

28 July 2019 1,579 3 View

Detecting gene copy number variation using RT-PCR?

Hi everyone, I am conducting an experiment to detect copy number variation among my gDNA samples using RT-PCR. I have generated standard curve for the genes of my interests and two controls...

23 July 2019 6,930 3 View

Fixable Viability Dye With Low Cytotoxicity?

Dear fellow scientists, I'm looking for a fixable viability dye for flow cytometry. Because I'm doing a time sensitive T cell stimulation assay, I need to add the viability dye before starting the...

09 July 2019 4,877 4 View

How to quantify amyloid load of IHC staining images with Image J?

Hi, everyone, I would like to quantify the Abeta load of my IHC staining images. How should I do it? someone use plaque area %, someone use plaque numbers, which one is better? And can I do it...

15 February 2019 2,109 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

No title

17 February 2021 4,217 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How can I spin a thick layer of organic polymer?

I need to spin a 120um thick film. My current procedure consists of spinning the polymer (about 20um), uv exposure, plasma etch, and then spinning another 20um thick layer, uv again, plasma etch...

10 February 2021 2,055 6 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View