Can I use snap frozen adipose tissue for ex-vivo lipolysis assay?

More Afsana B Trini's questions See All

What issues should be considered for the formation of complete shell structure while synthesizing core-shell structured nanoparticle?

As a new student researcher I've to reproduce the core-shell structured NPs that is already developed by another researcher. Although I'm following the same protocol, but In my case, for some of...

17 September 2023 9,419 1 View

How to prepare 50mM 10mL HEPES buffer from 1M HEPES buffer solution?

I tried to make HEPES buffer solution by using C1V1 = C2V2 formula. But the prepared buffer isn't okay. could anyone please suggest me how to prepare 50mM 10mL HEPES buffer from 1M HEPES buffer...

30 July 2023 6,963 10 View

Which "Defect Density" and range of defect density value is discussed properly?

I am working with solar cell simulation. So can I get some paper in which "Defect Density" and range of defect density value is discussed properly? I mean what is the range of the defect value?...

14 December 2021 6,520 3 View

Is there any range of defect density value?

My thesis work is based on simulation of solar cell in scaps 1D. In scaps I add a defect in individual layer. Is there any range of defect density value? I mean i need a reference paper in which...

13 December 2021 4,132 5 View

Can anyone tell me how to simulate GaInP in scaps 1D?

I tried GaAs solar cell..But i have some confusion fir GaInP

19 October 2021 1,853 6 View

I am working on GaAs solar cell i tried to simulate it in Comsol & Scaps. But i find Can anyone tell me how can i get this right?

I tried simulate the GaAs same as Si in comsol..But i couldn’t get any result.Similar with the SCAPS

13 July 2021 8,202 3 View

What can be the suitable method for measuring adipose tissue T3 and T4 level?

We want to measure adipose tissue level of T3 and T4. According to the literatures, tissue levels of these are mainly measured by RIA. For serum samples, researchers are also using LC-MS/MS. I...

01 August 2017 5,527 4 View

Superscript IV first strand synthesis kit (Invitrogen)is giving me poor quality of cDNA. Does anyone use it?

Hello, I am using Superscript IV first strand synthesis kit for making cDNA from RNA samples. The 260/280 and 260/230 values of My RNA samples are above 2. However after reverse transcription with...

14 February 2017 5,788 1 View

Hi.... Can anyone help with a protocol to infect a THP1 and Jurkat cell line with C.neoformans?

trying to infect cell lines with C.neoformans to look at uptake of ions

05 June 2016 7,619 4 View

I need articles for pharmacology glossary that defines, selective toxicity, therapeutic index, pharmacokinetics, efficacy and diuretics?

Would you please help me out finding an articles for each glossary what it means in terms of pharmacology?

31 March 2016 2,679 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View