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Questions related from Afsana B Trini
Our lab mass spec is API 4000 (which does not have in built diverter valve like 5500 series) and the LC system is Agilent 1100. We are planning to incorporate a diverter valve in between the...
08 October 2017 3,875 5 View
We want to measure adipose tissue level of T3 and T4. According to the literatures, tissue levels of these are mainly measured by RIA. For serum samples, researchers are also using LC-MS/MS. I...
02 August 2017 5,551 4 View
I am planning to measure ex-vivo lipolysis in inguinal adipose tissue of rats. We collected the tissues and immediately snap-frozen in liquid nitrogen. The papers normally recomment to preserve...
24 May 2017 2,963 2 View
In type 2, deiodinase (Dio2 or D2) assay, tissue homogenate is mixed with radiolabeled thyroxine and other cosubstrates. The resulting radiolabeled iodide is measured and used to determine the...
04 May 2017 5,050 2 View
Hello, I am using Superscript IV first strand synthesis kit for making cDNA from RNA samples. The 260/280 and 260/230 values of My RNA samples are above 2. However after reverse transcription with...
15 February 2017 5,810 1 View
I am working with adipose tissues. I normally make cDNA using 'High capacity cDNA RT kit' (Thermofisher). For making standard, I dilute my cDNA by 10 fold with Tris HCL buffer which is my...
12 December 2016 2,256 9 View
