Can i use RIPA buffer as nuclei lysis buffer for chip?

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How can I measure the quantity of hydrocarbon based material (i.e., paraffin oil) in a soil sample?

I aim to measure the concentration of hydrocarbon based organic compound from a certain quantity of soil sample. I would really appreciate it if anyone can give me some experimental guidelines.

07 January 2024 2,549 1 View

Give some research topic in oceanography related with arc-gis ?

I want to know about some research topic for oceanography which is related to arc-gis.

10 July 2023 7,892 1 View

Does crystalline polymeric SnCl2 become monomeric after getting dissolved in THF ?

In the solid state, crystalline SnCl2 forms chains linked via chloride bridges. If I dissolve this crystalline SnCl2 in THF , then what will be the probable outcome ? Will it break the polymeric...

04 May 2023 4,672 0 View

What is the best method for a two solvent crystallization ?

My compound is soluble in THF ,DCM and Toluene .I am doing small scale crystallization in 5 ml vial taking 10 -20 mg of solid compound in each vial. I have already tried THF, THF/Pentane, DCM,...

05 April 2023 9,260 3 View

Can anyone help me to find any download link of Silvaco 'Victory Device Simulator' software?

Victory Device simulator of Silvaco is generally used for simulating devices with charge (ion, atom) transport mechanism.

11 January 2023 4,286 0 View

Can I simulate any RRAM device in Silvaco or Sentaurus TCAD? If Yes, then how can I define the RRAM device properties in TCAD?

I am actually interested to simulate the RRAM devices in TCAD to investigate the capability of multi-bit functionality of RRAM devices. But I have no idea about whether a MIM device can be...

09 January 2023 6,313 2 View

Which equipment can help in measuring the swelling of pouch cells digitally ?

I am using 1900 mAh pouch cells for the testing. Feel free to ask further details related to the questions

28 December 2022 7,801 0 View

How to confirm that a lithiation reaction has reached 100% completion ? How to reduce the rate of side product formation during lithiation reaction?

I do lithiation of a aryl bromide having Phosphine group in the moiety . But even if I add little excess of of nBuLi then also 100% conversion is not happening . The nBuLi bottle is new. There is...

01 December 2022 6,733 4 View

Can chemical treatment of natural fibers reduce tensile, flexural or other mechanical properties of fiber reinforced polymer composite?

Chemical treatment is done to ensure better fiber-polymer bonding by diminishing water absorbing tendency of natural fibers. Good mechanical properties are expected after this operation....

28 August 2022 9,262 3 View

How the modulus of elasticity of natural fiber reinforced hybrid polymer composite will change with increasing volume percentage of fiber?

I have found mostly the modulus of elasticity gradually increases with fiber percentage. If it is a must, then why does it happen? Is the reverse of it, I mean, gradually decrement of modulus...

30 April 2022 9,770 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View