can i use RIPA buffer as nuclei lysis buffer for chip analysis instead of 1% SDS lysis buffer? then what will be the advantage and disadvantages.
Yes, but there are better buffers to use. See my paper in PNAS for a better buffer for ChIP.
yes, however, RIPA has some pH fluctuation problem
thanks Leyma De Haro and Kausik Majumder for all the informations you have provided.
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