Yan et al., 2014. in their paper "A G-quadruplex DNA-based, Label-Free and Ultra sensitive Strategy for microRNA Detection" (paper is attached with this post) suggested a ultra sensitive method of miRNA detection with the help of n-methyl mesoporphyrin(NMM), g-quadruplex DNA (GQD) and DSN enzyme. The interaction of the GQD and NMM led to a label free signal output. In this experiment author used f-7000 spectrometer from Hitachi, Japan.
Unfortunately in our lab, we do not have F-7000 fluorescent spectrometer or any likely instrument. What we have a small bench top flurometer from invitrogen, Qubit 2.0.
Can I perform the same experiment using qubit 2.0?
Protocol: Fluorometric Analysis- All fluorescence measurements were performed on an F-7000 spectrometer (Hitachi, Japan). The instrument settings were as follows: λEX= 399 nm (bandpass 10 nm), λEM from 550 nm to 700 nm (bandpass 10 nm) and the photomultiplier tube (PMT) detector voltage = 500 V.
Incubation were performed on a PCR Amplifier (Yan et al., 2014, Scientific Reports, 4:7400).
If qubit does not work zoo can try to use a TECAN plate reader. Best wishes
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