Leonardo Gatticchi

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Questions related from Leonardo Gatticchi

It is possible to increase genomic DNA yield from mouse hepatocytes by DTT addition?

I am using DNeasy Blood & Tissue Kit (50) cat#69504 to isolate genomic DNA from mouse hepatocytes and I am wondering about the exact composition of buffer AL. Although I can get good genomic...

06 June 2015 8,804 4 View

Does anybody use Amaxa nucleofector to transfect primary mouse hepatocytes? Can someone suggest me a protocol?

I'm trying to transfect freshly isolated mouse primary hepatocytes with specific Amaxa nucleofection kit. Hepatocytes are viable after isolation but they die shortly after transfection and I can't...

02 February 2015 3,750 3 View