Hi, I have natural polysaccharides and their modified derivatives. I want to test them for antibacterial activity but some of samples are too viscous at 10mg/ml that they don't get into the tip of pipette , I cannot load them properly into well. Most of these viscous samples reduce their viscosity in saline solution. So I was wondering that can I use normal saline 0.9% as solvent to disperse my samples for antimicrobial studies ?
Dear Yasir Iqbal ,
I am afraid that using sodium chloride aqueous solution to disperse polysaccharides will not achieve good results. As far as I know, using NaCl solution to dissolve certain polysaccharides is the way to make stable hydrogels of these polysaccharides. In other words, this may cause your experiment results to go in another direction. I think you can reduce the concentration of polysaccharides to reduce viscosity.
Best regards,
Weihao
@Phil the samples are too viscous And I want to check them at 10-40mg/ml range. So I want to use normal saline. Should I consider this situation as a need to use ?
I have seen that the Phil Geis viscosity of polymer that I am working on reduces in saline solution, so i came up with the saline idea. Yes actually its hydrocolloid swells even at low concentrations,
Of the choices beyond water, phys saline is prob the best. But think you should find another method. Dynamics of solubility, diffusion through agar matrix, medium/salt comp choice, etc. can screw up diffusion/solubility of a complex polymer. Why not just use serial dilution in minimal medium?
@Phil Sir thank you for your detailed elaboration, I will use a serial dilution
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