My study involves establishing chronic nicotine addiction in mice and I would to check the cotinine level in mice urine using HPLC but currently protocols I found are done using human urine. Bioassays and GC are expensive, and I would like to use urine as the sample. Thus, how do I modify the HPLC protocol for mice urine?
@KienVu thank you for your suggestions! I had came across this paper too. It's very thoroughly written, but yes, it's using human sample.
Is there any way to isolate the cotinine from the mice urine first and then quantify?
What about HPLC method used for rat urine? Is it comparable to mice? I found an old paper😅
I am new to mice studies, but I was told there are reference values from studies with human sample that can be used to calculate for animals. I haven't come across any papers that explain so. I have worked with zebrafish before, but they have very good database that explain differences between zebrafish and human, in which, for some strange reason I can't find for mouse.
I have no practical experience, however, I hope the provided link will be very much helpful for you. Please have a look on the following link:
http://163.15.154.123/retrieve/3410/715009-3.pdf
Article A Simple, Sensitive, and Rapid Method for the Determination ...
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