Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Which root in this tap root system, in the picture attached, should be used for root sectioning in order to study the root anatomical details?

There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...

14 February 2021 1,816 3 View

Hi, i am working on PARP inhibitors poorly water soluble but soluble in toxic solvents acetonitrile / DMSO, please suggest nontoxic solvents ?

Drug is poorly water soluble and soluble in solvents like acetonitile , DMSO, mixture of methanol and water but they are toxic. kindly suggest any safe solvent.

18 September 2020 5,128 4 View

How do I conclude if my gene has low expression based on the results obtained?

Hello all, I am working on genes affected in cholesterol metabolism in patients with Coronary Artery Disease and PCSK9 is one such target gene in my study. I have managed to obtain stable results...

13 June 2020 7,074 9 View

What is the institute/organization that provides training for hydroponics development ?

I also want to know the organization working on development of nutrient solution for hydroponics and the organization providing training on it.

02 June 2020 8,901 4 View

What method can I use to analyse the language on Social media?

I'm doing a comparative study on social media language used by native and non-native speakers with special reference to Instagram. I am planning on using Discourse analysis. What is your take on...

18 April 2020 300 25 View

In biosorption process (Lantana camara leaves as biosorbent), why do we get a precipitate after putting the filtrate on hot plate for agitation?

Usually, we get a clear filtrate after performing batch experiments; same is with Lantana indica leaves as biosorbent. But, after filtration we observe dark precipitation in this case. Is it due...

03 April 2020 9,406 1 View

Are there reserach papers on detecting whole multivariate time-series as anomalous from a set of multivariate time-series?

Consider a scenario with Dataset D as {T1, T2, ..., Tn} and Ti is a multivariate time-series of length mi as {X1, X2, ..., Xmi}. Here each record of the time-series Xi is a vector of attribute...

05 February 2020 967 2 View

Procuring AZ81 alloy ?

Dear Ma'am, I am interested in working with Mg-alloy since it is highly flammable, I am not able to procure the material nor create the composition of AZ series in the foundry. kindly help me...

12 January 2020 2,365 3 View

Which organic solvent is best for phytochemical extraction of plant leaf material??

Describe selection of best organic solvent for extraction of phyto chemicals from plant leaf.

10 November 2019 2,389 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View