Ankita Vaishampayan

3 Questions 2 Answers 0 Followers

Questions related from Ankita Vaishampayan

Is the spectinomycin cassette the same in pDL277 and pDL278 plasmids?

Hi, I am trying to delete genes in Streptococcus mutans using PCR ligation mutagenesis following published primers of the target gene locus and Spectinomycin cassette. The study used pDL278...

08 June 2023 7,629 2 View

Transformation of a plasmid into S. aureus?

I am trying to transform a plasmid that I prepared with the backbone, spacer, and repair templates (approx 14Kb) into a strain of S. aureus (ATCC 6538). I tried preparing electrocompetent cells...

25 January 2023 5,815 4 View

Can I use expired sheep blood?

Hello, I need to revive the anaerobic bacterial culture of Corynebacterium durum. The protocol recommends using Tryptic Soy Broth and Sheep's Blood. I have a bottle of Sheep's Blood but that has...

29 June 2022 9,355 4 View