I've been having a bit of trouble with yeast contamination for DU145 cells. I purchased nystatin to rid the contam but nystatin will not be able to help with any potential bacterial contam. Is it possible to use both the AA solution and nystatin or just leave out the AA?
Hello Farzana Mohamed
Nystatin is both fungistatic and fungicidal in vitro against a wide variety of yeasts and yeast-like fungi. So, you may use a combination of antibiotic (Penicillin-Streptomycin) and nystatin solution. This combination will take care of bacterial, fungal as well as yeast contamination. Excessive use of these reagents may turn out to be harmful to the cells. So, as far as possible avoid unnecessary use of these reagents.
Best.
You can. But it's probably better to toss this flask and start with a fresh vial. And clean your incubator and hood thoroughly.
Yassir Zargar Thank you for the answer. Unfortunately, AA is ineffective against yeast contamination.
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