Can I use black 96 well plates with clear bottoms for Promega Caspase Glo Assay or does it have to be the white plates?

15 November 2022 1 4K Report

I am having a hard time getting results with Caspase Glo Assay after radiation. I am trying to troubleshoot what is going wrong.

Malcolm Nobre

Hello Aida Nourbakhsh

The Caspase-Glo assay is a luminescent assay. It consists of caspase cleavage of the substrate and generation of a glow-type luminescent signal, produced by luciferase. The luminescence so produced is proportional to the amount of caspase activity present. The white 96-well plates are well suited for this purpose. These plates are used to give maximum reflection as well as to minimize autofluorescence and auto luminescence.

The black 96-well plates are used when there is a specific need to minimize background in fluorescence reading.

Best.

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