Aida Nourbakhsh

4 Questions 1 Answers 0 Followers

Questions related from Aida Nourbakhsh

Can I use black 96 well plates with clear bottoms for Promega Caspase Glo Assay or does it have to be the white plates?

I am having a hard time getting results with Caspase Glo Assay after radiation. I am trying to troubleshoot what is going wrong.

15 November 2022 3,993 1 View

I keep getting lower signal in Promega Caspase Glo Assay after I radiate cells, what could be going wrong?

I have been radiating Schwann cells with different protocols and then do the Progema Caspase Glo Assay but don't see an increase in signal, as a matter of fact it is either equal to control or...

15 November 2022 6,305 0 View

Can Alkaline phosphatase be added to cell culture media to reduce ATP levels hence down regulate the activation of P2X2 receptors?

I am doing FM1-43FX staining with the addition of ATP and nonselective antagonist of P2x, 200 uM Suramin. I get robust inhibition of dye uptake in the presence of Suramin. However I get positive...

23 October 2020 337 2 View

How can I incubate animal tissue (ileum) with Actinomycin D in an organ bath to measure RNA decay?

I have tried to treat mouse ileum/colon tissue with Actinomycin D in an organ bath up to 12 hr time points. I used 18s as my housekeeping gene. When I isolated RNA with Trizol I got a very high...

21 April 2015 5,960 2 View