An author's institutional affiliation was different at the time of the study and while writing up, which one should be provided for publication?

All the researchers of a study have moved to a different institution. We conducted the research while we were at the old institution. Now, as we write-up the manuscript for journal publication,...

08 November 2020 285 3 View

Can anyone help with this doubt on sample characteristics in a prevalence study-regarding its homogeneity and generalizability?

Background-In a community-based prevalence study, sampling was done from different geographical regions using probability proportional to sample size method and random sampling of individuals...

28 July 2020 5,748 3 View

Can a study design and rationale paper (or a protocol) be written for publication after the study is completed?

My team has an afterthought on writing up the study design for publication after study completion. The study is completed and while writing up the results manuscript, we feel that the methods...

08 May 2020 5,230 11 View

Is it necessary to prepare all the biological replicates on the same day?

I am wondering whether it is completely necessary to have a biological replicates for an RNA seq experiment to be prepared on the same day. What if in case of a differential gene expression...

20 September 2018 1,825 5 View

Problem with topo cloning strategy.

29 December 2016 9,460 6 View

If the ground state dipole moment is 11.2 Debye, what is the best way to calculate excited state dipole moment?

Initially ground state dipole moment was calculated from the theoretical method using software. Due to lack of facilities I can not calculate Excited state dipole moment. Seeking to calculate...

09 September 2013 2,173 8 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View