Can I use a different unlabeled DNA probe as a specific competitor to the labeled DNA probe when performing EMSA?

06 June 2016 1 8K Report

I haven't got an unlabeled DNA probe of the same labelled (biotin) probe. I was wondering if it is mandatory to use the same unlabeled probe or can I use a different probe, which I am certain that my protein will bind to?

Thanks,

Kiran :)

Kiran Sreeja Jayan

Thanks Alice :)

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