I have extracted the bacterial genomic DNA using Roche High PCR Template preparation kit. After elution with elution buffer, I had a white pellet like stuff! I inverted the tubes a couple of times and it just dissolved! The centrifuge balance tube (water) also had this white stuff. I repeated the extraction but this time eluting with water and I had the white stuff both in my centrifuge balance (just water) and eluted DNA. I was wondering if anyone had a similar experience? I don't think it is salt because my balance had just water in it.
And what kind of water? Is it ultrapure, distilled, tap water...? Are you using filter-tips when pipetting?
Hi Galo Adrian Goig,
Yes, I was using Milli-Q water. No, I wasn't using filter-tips.
I had the same experience when I worked with Roche High Pure Viral Nucleic Acid Purification kit.
I suspect that silica resin escape from spin column and during centrifugation pellet on the bottom of the tube. But I am not sure about it.
I thought about it, but my colleague told me he dint have any such problem (I used his kit for extraction). I did a phenol:chloroform extraction followed by ethanol precipitation. Still I can see traces of the stuff. I am planning to do a PCR tomorrow. Hopefully, there will be no issues.
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