01 February 2021 6 927 Report

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx

I've got around 500-600ng of DNA in a total volume of 35ul.

Would I get satisfactory results with this amount of DNA for cloning purposes?

If not, do you have another suggestion?

Will I lose a lot of DNA, If I do ethanol precipitation on my sample?

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