I have 500ul of sample from where I try to precipitate proteins.The sample is 2% SDS and very dilute. The conventional chl-methanol protocol suggests that I use
1:3:1:4
volumes (sample:methanol:chloroform:water) to precipitate sample.This makes the procedure uncomfortable since the volume is very large.
My question is can I instead treat the samples as if 4 volumes of water were part of the sample, so rather than doing
500:1500:500:2000
can I instead do:
500:375:125:0? Has anyone tried that?
Jarosław Króliczewski
Why don't you use the TCA precipitations method ? You can also concentrate your protein by Centrifugal Filter Unit i.e Millipore.
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