Pavel V Shliaha

9 Questions 2 Answers 0 Followers

Questions related from Pavel V Shliaha

Is there a published method for denaturing protein SEC?

I would like to perform protein fractionation similar to the results of SDS-PAGE, but using an LC system and SEC column. Has anyone ever done smth similar and visualised fractions on a gel?

20 October 2022 9,632 3 View

Are there any papers on packing 300um columns for LC-MS/MS?

I would like to run LC-MS/MS with a 300um column, but the commercially available ones are only 15 cm. There are many papers that discuss packing 50-150um columns in 365um OD capillary, but has...

11 March 2022 2,440 1 View

Is there an efficient way to quickly collect small number of cells after FACS?

I am trying to do proteomics of rare cells populations and I need to collect say 100K cells from 5ml of media after FACS. I am looking for a simple and efficient method, preferable not based on...

30 September 2019 7,390 3 View

Is there an algorithm prediction for peptide absorbance at 214nm?

I have a peptide I would like to quantify with 214 nm abrobance. Is there an algorithm (or a rule of thumb,) to predict what the absorbance should be like there for 280nm?

16 April 2019 7,390 1 View

Is there a his-tagged protein standard for IMAC HPLC?

I am doing some his-tagged protein purification and I would like to see if the chromatography works by running a standard. Is there a cheap histidine tagged protein people tend to use? Also the...

08 April 2019 9,404 4 View

How to make filter tips from normal tips?

I need to get a filter of good porosity (flows well) at the bottom of a 200ul tip. I need to filter small volumes of solutions in 96 format. Any ideas? The filter needs to be inert (so C18 or C8...

30 January 2019 8,434 3 View

How to stop evaporation in speed vac at a particular volume?

I am doing lots of proteomics and hence I often times perform speed vac. I do not want to dry my samples completely, but I want a certain fixed volume left (lets say 100ul). Has anyone found a way...

10 April 2018 7,635 4 View

Can I treat protein sample as part of the water phase with chloroform-methanol protein precipitation?

I have 500ul of sample from where I try to precipitate proteins.The sample is 2% SDS and very dilute. The conventional chl-methanol protocol suggests that I use 1:3:1:4 volumes...

26 April 2017 9,981 1 View

Offline fraction of peptides by HILIC using disposable columns?

I need to perform offline fractionation by HILIC. I have done offline fractionation using disposable RP columns (I have used home made columns with oligo R3 material, but Thermo sells disposable...

07 November 2016 4,854 1 View