12 Questions 5 Answers 0 Followers
Questions related from Pavel V Shliaha
I would like to perform protein fractionation similar to the results of SDS-PAGE, but using an LC system and SEC column. Has anyone ever done smth similar and visualised fractions on a gel?
20 October 2022 9,677 3 View
I would like to run LC-MS/MS with a 300um column, but the commercially available ones are only 15 cm. There are many papers that discuss packing 50-150um columns in 365um OD capillary, but has...
11 March 2022 2,490 1 View
I am trying to do proteomics of rare cells populations and I need to collect say 100K cells from 5ml of media after FACS. I am looking for a simple and efficient method, preferable not based on...
30 September 2019 7,498 3 View
I have a peptide I would like to quantify with 214 nm abrobance. Is there an algorithm (or a rule of thumb,) to predict what the absorbance should be like there for 280nm?
16 April 2019 7,454 1 View
I am doing some his-tagged protein purification and I would like to see if the chromatography works by running a standard. Is there a cheap histidine tagged protein people tend to use? Also the...
08 April 2019 9,469 4 View
I need to get a filter of good porosity (flows well) at the bottom of a 200ul tip. I need to filter small volumes of solutions in 96 format. Any ideas? The filter needs to be inert (so C18 or C8...
30 January 2019 8,488 3 View
I am doing a lot of peptide synthesis work and use the following reagents: 1) 20% piperidine in DMF 2) 0.65M HBTU in DMF 3) acetic anhydride in DMF 4) N-Methylmorpholine in DMF I noticed that...
03 July 2018 8,600 4 View
I am doing lots of proteomics and hence I often times perform speed vac. I do not want to dry my samples completely, but I want a certain fixed volume left (lets say 100ul). Has anyone found a way...
10 April 2018 7,714 4 View
I have 500ul of sample from where I try to precipitate proteins.The sample is 2% SDS and very dilute. The conventional chl-methanol protocol suggests that I use 1:3:1:4 volumes...
26 April 2017 10,035 1 View
I need to ship drosophilla sample for a proteomics study (drosophilla heads). Will dry ice be ok? Has anyone succesfully shipped intact drosophilla or drosophilla heads for proteomics studies?
28 January 2017 4,345 4 View
I need to perform offline fractionation by HILIC. I have done offline fractionation using disposable RP columns (I have used home made columns with oligo R3 material, but Thermo sells disposable...
07 November 2016 4,935 1 View
We have got an opentrons robot and installed it and I diligently read all the API opentrons documentation and took some python courses. Hence in principle I could write any sort of script, but...
01 January 1970 735 0 View
