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Questions related from Dina Mostafa
I am staining pancreas with HE. I found beta cells show pale hematoxylin staining in conditional knockout mice compared to surrounding other islet cells, exocrine and acinar tissue " this means...
04 April 2018 305 1 View
I want to ask about normalization step: if I want to normalize by DNA content how I should isolate DNA after treatment of the islets with acidified ethanol?
08 August 2017 4,459 0 View
Recently, I am trying to isolate islets from db/db mice. I use the same protocol I regularly use for islets isolation from mice but I can't get any islets or very few pale islets attached to...
02 February 2017 5,939 7 View
I am recently extracting murine islets. I see most researchers recommend pooling islets from several mice. I just want to check expression of my protein of interest in islets as an initial stage...
11 November 2016 9,381 3 View
I have been using tubulin as internal control for my western blotting analysis of mice islet lysates. I wonder if there is a better internal control the researchers working with mice islets use.
10 October 2016 6,274 3 View
If this works what kind of tranfection reagent is recommended for the islets?
08 August 2016 4,508 9 View
I am interested in knowing the expression of a certain protein in the islets under feeding and fasting conditions. I use the same total protein concentration for immunolotting. In my first trial I...
08 August 2016 4,321 1 View
I have a mouse model that expresses modified Cre recombinase, so that only upon tamoxifen treatment of the animals they express Cre, thus KO of floxed gene can be induced. I want to isolate the...
08 August 2016 2,687 2 View
I did a glucose stimulated insulin secretion assay for MIN6 cells. I measured the released insulin in the Kreb's Ringer buffer containing certain concentrations of glucose. In the end I collected...
07 July 2016 3,898 2 View
I want to transfect PGL3 plasmid to MIN6 cells. I tried Trans-it trnsfection reagent but didn't work well.
01 January 1970 5,293 2 View