Dina Mostafa

10 Questions 14 Answers 0 Followers

Questions related from Dina Mostafa

What is the meaning of pale stained nuclei with hematoxylin?

I am staining pancreas with HE. I found beta cells show pale hematoxylin staining in conditional knockout mice compared to surrounding other islet cells, exocrine and acinar tissue " this means...

04 April 2018 304 1 View

Normalization by DNA content in glucose stimulated insulin secretion assay for islets?

I want to ask about normalization step: if I want to normalize by DNA content how I should isolate DNA after treatment of the islets with acidified ethanol?

08 August 2017 4,457 0 View

Has anybody faced difficulties with islets isolation from db/db mouse model?

Recently, I am trying to isolate islets from db/db mice. I use the same protocol I regularly use for islets isolation from mice but I can't get any islets or very few pale islets attached to...

02 February 2017 5,937 7 View

For murine islets, can I work on islets extracted from one mouse or I have to pool from several mice?

I am recently extracting murine islets. I see most researchers recommend pooling islets from several mice. I just want to check expression of my protein of interest in islets as an initial stage...

11 November 2016 9,380 3 View

What is the best internal control for western blots of mice lysates?

I have been using tubulin as internal control for my western blotting analysis of mice islet lysates. I wonder if there is a better internal control the researchers working with mice islets use.

10 October 2016 6,273 3 View

I am asking if somebody previously transfected islets of Langerhans isolated from mice with siRNA before. Is this going to work?

If this works what kind of tranfection reagent is recommended for the islets?

08 August 2016 4,505 9 View

Are beta actin and tubulin levels in islets affected by fasting ?

I am interested in knowing the expression of a certain protein in the islets under feeding and fasting conditions. I use the same total protein concentration for immunolotting. In my first trial I...

08 August 2016 4,319 1 View

Can I treat mice islets expressing tamoxifen induced cre in vitro with tamoxifen to knock out a floxed gene?

I have a mouse model that expresses modified Cre recombinase, so that only upon tamoxifen treatment of the animals they express Cre, thus KO of floxed gene can be induced. I want to isolate the...

08 August 2016 2,685 2 View

I want to ask about measurement of insulin content in MIN6 cells?

I did a glucose stimulated insulin secretion assay for MIN6 cells. I measured the released insulin in the Kreb's Ringer buffer containing certain concentrations of glucose. In the end I collected...

07 July 2016 3,896 2 View

Which transfection reagent is best with reporter assays on MIN6 cells?

I want to transfect PGL3 plasmid to MIN6 cells. I tried Trans-it trnsfection reagent but didn't work well.

01 January 1970 5,291 2 View