I have been using tubulin as internal control for my western blotting analysis of mice islet lysates. I wonder if there is a better internal control the researchers working with mice islets use.
hi
routinely beta-actin & GAPDH is ok .
good luck
http://www.abcam.com/primary-antibodies/loading-control-guide
Hi, check all of the following links having excellent knowledge to choose the best reference internal control for western blots.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0043028
http://www.abcam.com/primary-antibodies/loading-control-guide
https://www.bio-rad-antibodies.com/western-blot-loading-controls-antibodies.html
https://www.labome.com/method/Loading-Controls-for-Western-Blots.html
https://www.novusbio.com/antibody-news/antibodies/the-importance-of-beta-actin-and-gapdh-loading-controls
http://www.bioscience.co.uk/userfiles/pdf/Western%20Blot%20Normalisation%20Application%20Note.pdf
For me they helped a lot in terms of understanding, hope would be helpful for you too.
But a fact which actually makes life somewhat easier on a stain free gel and on the blot is that try to select the internal control which has a wide difference with your protein of interest in terms of molecular weight. This pronounced difference is really helpful many times.
