To isolate DNA from mouse ear pieces for genotyping, we use a digestion buffer and we add prot K. Our DNA digestion buffer contains: Tris-HCl (pH 8.0), EDTA (pH 8.0), NaCl and SDS. We always add the prot K at the moment of DNA isolation, but of course we make a bit more than needed. Can we store this, and re-use this for the next DNA isolation? Thanks!
Marc M. Abitbol
It is always better tu use fresh solutions or reagents if we have any doubt about the outcome
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Ganapati Bhat
You can store it but SDS should be avoided. I usually make buffer solution with out SDS and with Prot K. Add the SDS at time of making tissue lysate. Mine is almost a year old kept in 4C still works fine.
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