Caro Bos

2 Questions 2 Answers 0 Followers

Questions related from Caro Bos

Can I use a PCR clean up kit on a PCR sample with loading dye?

My PCR products are probably specific (e.g. only 1 band), so usually I use a PCR clean up kit to purify my PCR sample (I only check 2 ul of the PCR product with loading dye on an agarose gel)....

27 June 2017 3,252 12 View

Can I store DNA digestion buffer including Prot K (e.g. at 4 or -20 degrees), or should I always add the latter fresh?

To isolate DNA from mouse ear pieces for genotyping, we use a digestion buffer and we add prot K. Our DNA digestion buffer contains: Tris-HCl (pH 8.0), EDTA (pH 8.0), NaCl and SDS. We always add...

09 August 2016 6,381 3 View