Does anyone have experience in the use of mycorrhiza in subsurface vertical flow constructed wetland in order to improve reed root groth?

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Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Hot to do MTT assay data analysis?

I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...

08 July 2024 1,900 1 View

What is wrong with Site-Directed Mutagenesis?

I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...

24 June 2024 6,079 4 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

How do you add multiple nodes or cutting planes in Abaqus?

Hi, In a previous question I asked how to effectively model springs in Abaqus. However, I figured out the feature under Assembly -> springs/dashspots -> node to node Springs. I have a cylinder...

20 June 2024 892 3 View

How do you add radial spring elements in Abaqus?

Hi, I am trying to complete a buckling analysis on a tube with an external pressure. This solves itself easily by adding radial constraint on the internal edge of the tube, one axial constraint...

17 June 2024 8,165 7 View

Given that Mesozoic placentals were largely arboreal (with fingers), do you know of any studies that illustrate the finger-to-hoof transition?

I'm interested in the earliest days of placental terrestrial locomotion in large herbivorous taxa. Phenacodus seems to be transitional in this regard. Any others?

06 June 2024 5,182 2 View

How reliable is ninhydrin test in solid-phase peptide synthesis?

Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...

21 May 2024 9,035 0 View

How does one generate a normalized TCSPC curve from actual photon counts versus time?

Sometimes TCSPC curves are analyzed using the actual photon counts, and sometimes they are normalized with the signal running from 0 to 1, or even from 0 to some value less than 1. What quantity...

20 May 2024 4,277 0 View

Problem when running Spin-Orbit Coupling using CASTEP. What is nlpot_prepare_precon_ks?

I am currently using CASTEP version 22.11, and I encounter the following error when running calculations with spin-orbit coupling: Error with dsytrf in nlpot_prepare_precon_ks Current trace...

19 May 2024 4,236 5 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

A question about arbuscular mycorrhizal???

How long it takes for arbuscular mycorrhiza to establish and produce benefits under experimental conditions？

25 July 2024 5,208 2 View

How to identify wetland area in Landsat imagery?

The indexes NDWI and MNDWI are use to identify waterbody. Can I use these index to identify wetland also? Thank you for advance.

25 July 2024 6,242 2 View

Granger causality construction for VECM model?

Dear people,

24 July 2024 1,252 2 View

RNA isolation from plant roots infected with both bacteria and fungi. How to isolate RNA from all of them simultaneously?

I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...

09 June 2024 9,876 0 View

I'm looking for publications about free water surface constructed wetlands conducted in the USA.?

I'm looking for publications about free water surface constructed wetlands conducted in the USA. Specifically, I'm interested in studies that focus on their performance in nutrient removal and the...

30 May 2024 5,880 0 View