How do you annotate figures to show significance?

30 January 2021 1,008 3 View

How can peptides based on allele sequences be delivered to mice?

I am looking to measure allele-specific immune responses for specific antigens. After identifying the most prominent alleles in a population, I am looking to express them in mice and then measure...

03 September 2020 9,281 1 View

Missing values in R lme(): how to include observations with missings in the outcome variable?

Hello, I am struggling to understand how I best use the na.action options in R's lme function for a hierarchical linear model. I have a longitudinal data set with a two-level nested structure...

23 July 2020 2,327 4 View

How do I interpret extremely low values of Blomberg's K with low P values?

I have a set of trees and am using Blomberg's K to detect phylogenetic signal. I am finding that for several of them, my values for K are close to 0 (in the region of K = 0.001 - 0.004) which...

05 December 2019 6,379 1 View

Warning messages in Abaqus relating to general contact, tie constraints, and stringer beam elements - are they an issue?

Hi, I have a 3D model that I am using to model a shear connections in reinforced concrete. The model is using C3D8R elements for the concrete and B31 elements for the reinforcement, which are...

27 February 2018 8,169 3 View

Liquid culture colony PCR technique?

I need to do a pcr on a liquid culture of E.coli. I'm amplifying a 150bp region of gyrA, using primers that I designed. I'm using NEB Next Ultra 2 Q5 MM. For the reaction, I am using 5ul NEB, 0.5...

25 October 2017 5,995 5 View

I am currently dealing with article critique on learning theories.? This been my first time to do this what would you recommend for effectiveness?

I am taking Edu 831 (Learning theories), it involves article critique on use of different learning theories in research. How can I critique an article effectively and what does it involve?

08 October 2017 1,958 3 View

How can research on attitude change benefit government initiatives to encourage young people to develop more healthy attitudes towards alcohol?

How do you think research on the Theory of Reasoned Action (TRA) and the Theory of Planned Behaviour (TPB) influence government initiatives to encourage healthy attitudes and perhaps even change...

12 August 2017 2,479 3 View

I would like to store 10 uL aliquots of bacterial culture. What would be the best way?

I need to store 10ul aliquots of bacterial culture , would the best way be to put them in PCR tubes with some gylcerol (10ul 50%) at -80?

31 January 2017 2,848 3 View

Unusual pelleting with ethanol precip after phenol chloroform. Help troubleshoot?

I've been using phenol chloroform extractions on my Chromatin IPs, but after the ethanol precipitation I either get very tiny pellets, or a smear along the side of the tube. I think it is...

07 June 2016 10,024 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View