Can I run Western blot after running native PAGE?

09 September 2017 0 716 Report

my target protein is 70 kd.which Acylamide percentage can I use? which method can I use. Can I use semidry blotter?

thank you very much for your answer.

Badges
Science topic

More Jia Wen-juan's questions See All

How to reduce the material coming out of the torque rheometer?

A physical mix was in vitro evaluated on a torque rheometer. The composition was predominantly recycled polypropylene (PP), yet the melt flow index (MFI) was found to be excessively high....

25 June 2024 10,154 0 View

Who is the use of Generative Artificial Intelligence in Qualitative Research?

One of the most intriguing frontiers in contemporary research is the application of Generative Artificial Intelligence (GenAI) in qualitative research. This technological advance offers tools that...

13 June 2024 781 2 View

¿Que enfoque metodológico me recomiendan para abordar los gremios tróficos en un área natural de BST?

Me interesa investigar la distribución espacial de los gremios tróficos de aves en un área natural específica de bosque seco tropical, analizando la abundancia relativa de cada gremio en las...

12 June 2024 9,313 1 View

Laser for perovskite module?

Hi, I'd like to know if someone knows about a company where I can buy an affordable laser-scriber for perovskite films and another thin film for the fabrication of modules. Many thanks,

31 May 2024 2,841 0 View

Fluorescence spectroscopy: signal from water?

In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...

16 May 2024 6,266 5 View

Can I calculate the high and low spin of the material in Quantum espresso?

I would like to simulate the high and slow spin configuration of a metal oxide. I am using QE for it, but in my research, I found that this kind of calculus is not possible. Or if it is possible,...

15 May 2024 6,497 3 View

Does anyone have a protocol for MPs assessment in fresh snow?

Dear colleagues Could someone send to me an already validated protocol for microplastic analysis in fresh snow that includes sampling and analysis in the lab? I suppose there is no need to digest...

15 May 2024 2,378 1 View

How can technology help hospitalized children?

How do you think technology can help hospitalized children?

10 May 2024 6,508 2 View

What mode can confocal microscopy use to observe metal surfaces?

How can confocal microscopy observe cells stained on metal surfaces

28 April 2024 2,804 1 View

How can confocal microscopy observe cells stained on metal surfaces?

How to observe cells stained on metal surfaces using confocal microscopy,How to adjust the confocal mode

28 April 2024 3,103 0 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View