Jia Wen-juan

6 Questions 1 Answers 0 Followers

Questions related from Jia Wen-juan

Can anyone tell me the differences of native PAGE,reducing SDS-PAGE and non-reducing SDS-PAGE and their application?

I'm not sure whether my antibody can recognise the endogenous protein(with native conformation ) and I want to determine this by WB,which method can I use?

10 October 2017 5,056 0 View

Can I run Western blot after running native PAGE?

my target protein is 70 kd.which Acylamide percentage can I use? which method can I use. Can I use semidry blotter? thank you very much for your answer.

09 September 2017 700 0 View

How can I distinguish the antigen of antibody : folding protein or unfolding protein?

the homemade antibody of my target protein detected the localization is the ER and golgi. the chimeric protein(GFP) merges with golgi marker.I wonder the antibody recognizes unfolding protein,how...

08 August 2017 2,359 3 View

The endogenous localization of my protein is ER, while the stable transfection to 293 T or CHO-K1 is golgi,why?

the protein expresses specifically in brain and lung.

06 June 2017 9,935 5 View

How to count cells in different slide stained with different antibodies and calculate percentage ?naked eye or software,which software?

Immunofluorescence assay, my target protein locates both in ER and Golgi, I want to calculate the percentage in the two organelle.

06 June 2017 4,070 2 View

I detected two unequal concentrations of substrate with gradient concentration of inhibitor - can I fit a curve to obtain the Ki?

I have determined Km and Kcat of enzyme and want to determine Ki of an inhibitor,I just detected 2 different concentration of substrate with gradient concentration of inhibitor,can I use those...

01 January 2016 8,875 1 View