I am conducting insecticide degradation study in soil on insecticides containing fipronil, imidacloprid and bifenthrin as their active ingredient. I would like to run standard on UPLC/HPLC not from technical grade as they seems to be very expensive.
u can,..but it is not recommended... in long run it may affect ur HPLC system.. and also u may get different peaks in the standard itself as HPLC is very sensitive and ur standard solutions that are not of HPLC grade have impurities in them...
I filter my 'dirty' samples through a 0.45 um syringe filter before I put them in the HPLC vial and inject them; you can do the same for your standards. You should also use a guard column that is a 1 cm disposable cartridge of the same packing material, e.g. C18. This will protect your HPLC column from blockages. Your column supplier will have these.
You can use technical grade solvent for short run or preliminary analysis.
But for longer time run the high purity solvent are preferred. Using technical grade solvent can cause poor result outcomes as it also affects the column as well.
It is generally not recommended to run technical grade solvent. For on time analysis u can run but before run it should be filter through 0.2 µm filter.
It is not advisable to run technical grade solvents in HPLC/UPLC. Contaminants present in technical grade solvents might result in back pressure due to blockage, which leads to shut down of system. Prefer HPLC/UPLC grade solvents for analysis.
Everybody know "money does not spring as the grass", but your attempt is quite risky. Your economy rates can not afford the additional costs in maintaining your chromatographic column and your own LC equipment. Try new LC solvents suppliers, especially those most competitive. I am sure you get better values around at your amazing Malaysia. Nice elutions!!
I assume that you are using an external standard method to determine your compounds. In addition to the above mentioned comments, standard solutions with high purity are desirable for your analysis, as you get accurate results and minimise the risk of technical problems with the column and LC.
It is better if you have primary standards. if not then is it possible for you to arrange secondary standards. Without appropriate standards it is not possible to quantify the analytes in the samples.