Mandeep Kaur

6 Questions 12 Answers 0 Followers

Questions related from Mandeep Kaur

Can anyone recommend a protocol for ion pair reverse phase hplc?

I am looking for a protocol for ion pair reverse phase hplc using Luna C18(2) column for estimation of intracellular nucleotide precursors (e.g. UTP, UDP, ATP, AMP etc.). The major concern is that...

10 March 2014 7,967 4 View

How do you measure the enzyme activity in bacterial cells?

I want to find out the activity of certain enzymes in bacteria. I have seen papers in literature in which people have taken crude extract of cells and have done enzyme activity. Now, suppose...

31 January 2014 9,897 4 View

Is there any method for quantification/estimation of NAD/NADH using colorimetric method?

I know one kit but I am looking preferentially for some colorimetric analysis or quantification using fluorescence spectroscopy. Help regarding any of the methods would be appreciated.

20 January 2014 1,116 2 View

What range of NADH/NAD ratio changes are significant if we want to increase/decrease in production of certain metabolite in a bacteria?

If we want to relate changes in NADH/NAD ratio to increase/decrease in production of certain metabolite in a bacteria, then what range of ratio changes will be considered significant?

20 January 2014 8,458 4 View

What is the approximate range of concentration of NAD/NADH in bacterial samples?

03 January 2014 4,488 1 View

Adding reactions to already existing mfa model - can anyone help?

I need to add reactions to an already existing set of reactions used for metabolic flux analysis for a pathway. While doing that I am getting all random and unrealistic values for fluxes. What...

02 January 2014 8,413 4 View