Can i multiplex 2 genes and singplex the rest in same run and compare results?

More Basma Ali's questions See All

Is beta actin Rna stable?

in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?

08 September 2019 6,957 4 View

Is there a difference between Rna expressed from cell culture and that extracted frim tissues?

if i can able to detect the transcript cd36 from cells but not from brain tissue .'.expected to have it'. ciuld it have more secondary conformational chnges than from cells?..just IDK

08 September 2019 5,637 5 View

Regarding Morris water maze test, if mice showed thigmotaxis in most of hidden platform training days whether reveciving LPS r control?

Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?

02 March 2019 7,230 3 View

Nuclear protein PGC 1 alpha ? best way to extract from mouse brain ?

i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...

11 December 2018 1,983 2 View

If i add protein extract to sample buffer is there difference if i heated till 60 for 10 mins than at 95 for 5 mins?

10 mins at 60 degrees vs 5 min at 95 degrees

10 November 2018 714 7 View

Tips for large number of samples homogenization for rna extraction?

iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...

10 November 2018 4,157 1 View

Is it better to reverse transcripe 1or 2ug of sample from brain tissue?

brain tissue reverse transcription..

10 November 2018 2,274 0 View

I cant get protein signals using RIPA buffer for extraction of one brain hemisphere ? (myhous keeping ptn is cytoplasmic ptn beta actin ? no signal?

i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...

10 November 2018 5,897 6 View

Does alzheimer's disease affect novality seeking behavior in mice? or it's all about memory retrieval?

modified Y maze , would be glad if you posted advices /papers

09 October 2018 7,257 0 View

I have done water maze and for each mouse i calculated the path lenght and time to latency ..can you please help me about how can i exclude outliers ?

How can i exclude outliers and how to use my data? i would be grateful if you can post sources , advice thank you

09 October 2018 2,694 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Basma Ali

@Laurence Stuart Dawkins-Hall

@laurence Stuart Dawkins-Hall

Sven Reister

Hi,

as you are going to normalize to the HKG and you are just looking for relative abundances this should work. In general you need to check that the PCR efficiency is comparable between Duplex and signleplex reaction of the particular assays

should i check for 5 samples or if i did for one sample it would be okay?