Basma Ali

18 Questions 30 Answers 0 Followers

Questions related from Basma Ali

Is there a difference between Rna expressed from cell culture and that extracted frim tissues?

if i can able to detect the transcript cd36 from cells but not from brain tissue .'.expected to have it'. ciuld it have more secondary conformational chnges than from cells?..just IDK

09 September 2019 5,710 5 View

Is beta actin Rna stable?

in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?

09 September 2019 7,028 4 View

Regarding Morris water maze test, if mice showed thigmotaxis in most of hidden platform training days whether reveciving LPS r control?

Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?

03 March 2019 7,288 3 View

Nuclear protein PGC 1 alpha ? best way to extract from mouse brain ?

i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...

12 December 2018 2,038 2 View

Tips for large number of samples homogenization for rna extraction?

iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...

11 November 2018 4,216 1 View

I cant get protein signals using RIPA buffer for extraction of one brain hemisphere ? (myhous keeping ptn is cytoplasmic ptn beta actin ? no signal?

i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...

11 November 2018 5,957 6 View

Is it better to reverse transcripe 1or 2ug of sample from brain tissue?

brain tissue reverse transcription..

11 November 2018 2,331 0 View

Can i multiplex 2 genes and singplex the rest in same run and compare results?

i mean i will do qpcr i will multiplex my housekeeping taqman gene (pl) vic dye with another taqman gene and will do the rest in singleplex format ? can i compare other genes expression to...

11 November 2018 2,930 3 View

If i add protein extract to sample buffer is there difference if i heated till 60 for 10 mins than at 95 for 5 mins?

10 mins at 60 degrees vs 5 min at 95 degrees

11 November 2018 770 7 View

Does alzheimer's disease affect novality seeking behavior in mice? or it's all about memory retrieval?

modified Y maze , would be glad if you posted advices /papers

10 October 2018 7,300 0 View

I have done water maze and for each mouse i calculated the path lenght and time to latency ..can you please help me about how can i exclude outliers ?

How can i exclude outliers and how to use my data? i would be grateful if you can post sources , advice thank you

10 October 2018 2,751 4 View

I've measured my RNA sample conc. using Qubit and Using U.V double beam spectrophotometer .However cocentration was much higher i spectro than qubit?

Any help? which reading to trust ? if trusted the Spectro results i will load around 1ul or less from my Rna sample for reverse transcription but if i used qubit results i will load 20ul of Rna...

09 September 2018 6,633 7 View

Can i use taqman gene expressn assay to detect a house keeping gene then use another taqman assay with different dye for the same gene rest of samp ?

my gene expression assay is about to be alll conusmed , iam wondering if i can use taqman gene expression assay for same gene but it have a different dye for rest of my samples?and combine data

09 September 2018 9,749 2 View

After cDNA formation ? do i have to measure again concentration or is it okay to decide on 1:1 ratio from total RNA ?

And what amount cDNA is it recommended to use in q-pcr?

08 August 2018 7,552 8 View

What is the usual total RNA concentration that can be isolated from mice brain hemisphere without cerebellum??

using double beam spectro..i appreciate your advice

08 August 2018 9,049 0 View

Please what are the optimum western blotting conditions for PGC 1 alpha protein from brain ?

Please give me your experience in that aim in a total need for your advice

08 August 2018 9,032 1 View

What is the usual total protein concentration do you get per hemisphere of mice brain tissue please?

i usually use qubit to asses protein concentration

07 July 2018 8,762 4 View

How can mouse antimouse antibodies being produced ?

i can understand that rabbit can produce rabbit antimouse antibody but how can mouse produce antimouse antibody ?

07 July 2018 4,293 3 View