modified Y maze , would be glad if you posted advices /papers
in RTqPCR. if i have my target gene expression at ct 33 and beta actin at ct 17..i repeated and that's always the ratio..could my rna be degraded but i have good ct for beta actin how?
08 September 2019 7,057 4 View
if i can able to detect the transcript cd36 from cells but not from brain tissue .'.expected to have it'. ciuld it have more secondary conformational chnges than from cells?..just IDK
08 September 2019 5,748 5 View
Should i exclude them from analysis? does LPS iP injection increases Thigmotaxis behavior?
02 March 2019 7,313 3 View
i have been struggeling to xtract it by ripa buffer ..then itake supernatent and heat in1 :1 ratio with 2x sample buffer at 95 degrees for 5 mins ..do yu have any suggestions ? it' s large ptn.so...
11 December 2018 2,068 2 View
iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...
10 November 2018 4,251 1 View
10 mins at 60 degrees vs 5 min at 95 degrees
10 November 2018 805 7 View
brain tissue reverse transcription..
10 November 2018 2,356 0 View
i doubt i have a problem in my extraction procedures .. i homogenize my brain in RIPA buffer NACl 150 mM , 25mM tris , SDS 0.1%, Sdium deoxycholate 1%, Triton x 1%, edta 5mM ... i use it in 1ul...
10 November 2018 5,984 6 View
i mean i will do qpcr i will multiplex my housekeeping taqman gene (pl) vic dye with another taqman gene and will do the rest in singleplex format ? can i compare other genes expression to...
10 November 2018 2,960 3 View
How can i exclude outliers and how to use my data? i would be grateful if you can post sources , advice thank you
09 October 2018 2,773 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
