I have received the sequence of plasmid, I aligned it with the gene sequence on APE plasmid editor and it is matched. But when I performed vector screening on ncbi, the sequence is strongly matched to vector. Can I make probe with this plasmid by cutting with relevant enzyme??
Michael J. Benedik
It would appear that you have an insert of around 450nts cloned into your plasmid, based on the simplest explanation of the figure you attach. Does this sound like what you should have?
If the insert is correct then of course you can make a probe from it by releasing with appropriate restriction enzymes or by PCR amplifying the insert region.
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Muhammad Faisal
Yes Sir, I have insert of 460nts cloned into plasmid. Thank you very much 😊
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