Muhammad Faisal

7 Questions 3 Answers 0 Followers

Questions related from Muhammad Faisal

How can I remove vector sequence to make sure that the sequence I have received is correct?

I want to remove vector sequence. Which software can be used to do so?

26 December 2023 8,721 2 View

I'm extracting Plasmid using Solarbio Kit but the concentration is very low , I can't make probe with this concentration, what would be the reason?

I have tried few times but I'm getting low concentration, Anyone please suggest me how can I increase the concentration.

27 September 2023 2,999 7 View

I have extracted DNA by cutting this band (picture attached) but the concentration was very low, Also the gel was showing non specific bands?

Previously I was getting good DNA concentration for other genes, but for this gene my DNA concentration is very low, also the band was very thin and is and it is always showing non specific...

29 August 2023 8,543 0 View

Can I make probe with this plasmid sequence that is matched to gene on APE Plasmid editor but during vector screening on ncbi it is matched to vector?

I have received the sequence of plasmid, I aligned it with the gene sequence on APE plasmid editor and it is matched. But when I performed vector screening on ncbi, the sequence is strongly...

14 August 2023 1,926 2 View

After extracting plasmid I sent samples for sequencing but the sequence is contaminated with vector?

After transformation and inoculation, I did colony pcr and got good bands, I selected the good bands for plasmid extraction. But after plasmid extraction when I sent my samples for sequencing, the...

06 July 2023 2,512 5 View

What maybe the general methods to conduct research on HIF signaling in fin development in fish?

03 March 2023 9,327 2 View

What maybe the general methods to conduct research on HIF signaling in fin development in fish?

03 March 2023 5,540 2 View