DSS induced colitis - which DSS is the best?

hi, I want to induce colitis in mice. There are many kinds of DSS in Sigma. Which one would be the best? More over I found in other company much cheaper but there is only written that it is DSS -...

07 August 2015 8,732 1 View

Is my vector suitable for retroviral PT67 cells with lucipherase. Does anyone know?

I want to transduce my target cells with vectors: pLXSN/SV40 LT and pLPCX/hTERT as it was successfully done by Pan et al. (PMID:20620546) or Bian et al (PMID: 15973764) to achieve cells...

31 December 2014 3,103 1 View

How can I identify a gene promoter (Cis-regulatory element)?

I want to check if promoters of a few genes (from cattle) are active in the cells. I want to clone GFP under the promoters and transfect the cells with the vector. The problem is I don`t know the...

11 December 2014 9,222 3 View

Does anyone know how to calculate the exact amount of RNA in the sample where 260:280 and 260:230 are not perfect?

Let's say 1,6 and 1,5 respectively. I want to know how much RNA there is in the sample. Is there any equation to eliminate protein and phenol influence on RNA concentration calculated by NanoDrop?...

02 March 2014 5,040 3 View

How can I perform miRNA PCR?

How can I perform miRNA microarrays and real time PCR to validate microarray results? Can I design my own primers for miRNA? Is there any suggested database of miRNA? Can i use SYBR green or...

11 December 2013 9,304 4 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View