Piotr Bąska

6 Questions 6 Answers 0 Followers

Questions related from Piotr Bąska

DSS induced colitis - which DSS is the best?

hi, I want to induce colitis in mice. There are many kinds of DSS in Sigma. Which one would be the best? More over I found in other company much cheaper but there is only written that it is DSS -...

08 August 2015 8,781 1 View

Is my vector suitable for retroviral PT67 cells with lucipherase. Does anyone know?

I want to transduce my target cells with vectors: pLXSN/SV40 LT and pLPCX/hTERT as it was successfully done by Pan et al. (PMID:20620546) or Bian et al (PMID: 15973764) to achieve cells...

01 January 2015 3,170 1 View

How can I identify a gene promoter (Cis-regulatory element)?

I want to check if promoters of a few genes (from cattle) are active in the cells. I want to clone GFP under the promoters and transfect the cells with the vector. The problem is I don`t know the...

12 December 2014 9,273 3 View

Does anyone know how to calculate the exact amount of RNA in the sample where 260:280 and 260:230 are not perfect?

Let's say 1,6 and 1,5 respectively. I want to know how much RNA there is in the sample. Is there any equation to eliminate protein and phenol influence on RNA concentration calculated by NanoDrop?...

03 March 2014 5,086 3 View

How can I perform miRNA PCR?

How can I perform miRNA microarrays and real time PCR to validate microarray results? Can I design my own primers for miRNA? Is there any suggested database of miRNA? Can i use SYBR green or...

12 December 2013 9,350 4 View

Can I make a coculture of epithelial cells of one strain and dendritic cells of other strain?

Will there be any non physiological interaction?

11 November 2013 640 4 View