I purified a protein using a buffer containing glycerol, and I want to preform density gradient centrifugation. Is it possible to load the sample on a sucrose gradient? Will the glycerol interfere?
thanks
Twenty one rats received 1.2 mg/kg A substance (MW:294.98 g/mol) dissolved in 1% Tween 80 by oral gavage for 14 consecutive days. How should I dilute substance A? How can I calculate the dose to...
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I have tried to achieve a separation of the nucleus and cytoplasm by using RLN buffer+ NP40 (0.2%), and then proceeding with GeneMATRIX universal RNA purification kit (the same used for total RNA...
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Hello all, In my research I intend to purify ChlG (a membrane protein) from cyanobacteria using a protein tag. I want to elute the protein together with its interacting partners or complexes. I...
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I am looking into modeling photoresist thermal reflow processes and looking for a mathematical model that can give me the effect of time on the reflow process (thickness, curvature, ect.). Thank you!
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I have multiple thin layers on my substrate and I want to use HMDS to improve the surface tension before using photoresist. The issue is that the developer solution will remove it and for the next...
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Hello to you, I would like to know if someone here can explain to me the difference between the following models for urban microclimate analysis before I start researching each one myself: -...
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I conducted an I-V curve measurement for my SWCNT FET and received a very odd result that I can't understand. The measurement was done with DI water as gate dielectric under gate voltage between...
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As you know, in accordance with the first law of switching, the current in the branch with an inductive coil, and in accordance with the second law of switching, the voltage across the capacitor...
09 August 2019 7,689 17 View
How long it will take for a successful editing?
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I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
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Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
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Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
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Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
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Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
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Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
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I would like to calculate the formation energy of P2-Na0.67Fe0.5Mn0.5O2 based on DFT, what should I do step by step. Any help would be appreciated. Thanks.
29 July 2024 8,248 2 View