I want to compare the 6 phosphogluconate dehydrogenase amino acid sequence of three leishmania strains. I amplifies the gene using target primer sets. Now I want to check the polymorphism among the sequences and any variation in aminoacids.
You can use different softwares like Mega5 and DNAStar to check the polymorphism after alignment of your sequences with other highly sequences. You can also translate the nucleic acid sequence to amino acid by said softwares.