Lovlesh Thakur

3 Questions 8 Answers 0 Followers

Questions related from Lovlesh Thakur

How to perform flow cytometry analysis of T reg cells, CD4 and CD8 cells simultaneously in one sample of PBMC's?

I want to perform flow cytometric analysis of T reg cell (Both nTregs and Tr1) and CD4 and CD8 cell in PBMC's from patient blood samples. Can I perform the cellular analysis simultaneously? Please...

25 December 2016 5,777 6 View

Do I need to stimulate PBMC's before doing flow cytometry to analyse CD4 and CD8 cells?

I have isolated PBMC's using ficoll gradient method and cryopreserved them around 6 months back. Now I want to analyse the CD4 and CD8 cells percentage. Is it necessary to stimulate the cells or I...

23 December 2016 9,889 5 View

Can I get protein sequence from PCR product of any gene (nucleotide sequence)?

I want to compare the 6 phosphogluconate dehydrogenase amino acid sequence of three leishmania strains. I amplifies the gene using target primer sets. Now I want to check the polymorphism among...

01 July 2016 8,969 3 View