How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Cas9 in vitro clevage, no bands or bigger bands?

12 February 2020 8,781 1 View

How can I measure antioxidant capacity of cosmetic lotion?

Dear scientist: I want to measure the antioxidant capacity of cosmetic creams and lotions with the DPPH and ABTS methods, but I am not sure how to prepare the samples. Will dissolving them in a...

10 February 2020 3,597 4 View

Random sampling from a dataset, preserving original probability distribution, with R sample function?

Hi everyone, I have a question concerning random sampling from a dataset. I would like to extract random samples from a dataset. I thought the "sample" function in R could be a good and simple...

10 February 2020 8,061 2 View

Can you help me know if there is any provider of pressure based flow control system for microfluidic similar to OB1 MK3 of Elveflow in Mexico?

We are interested in developing a electrospinning system and need: Flow rate: 1-5 mL/h

04 December 2019 239 1 View

Discrete event pattern recognition in time series not equally distributed?

I have more than 400 different events that occur during two years, some of them can occur 4000 times an others no more than 50 times. These events are not equally distributed, e.g., one at...

20 November 2019 5,083 3 View

Is the use of GLM correct for Binary response?

From a large sample (RCT) I got a binary response X (yes/no) and would like to see if there is a difference using NIH grants which is also a binary response (yes/no) Using R I made this code...

25 June 2019 2,707 7 View

What is the best technology for concentrating seaweed extract?

I am studying how to concentrate an extract of algae obtained in an alkaline pre-treatment of red algae. The extract has a high conductivity (about 25 mS/cm) and we need to concentrate the organic...

14 May 2019 1,151 2 View

What suggestions could you give me to create a research question for my bachelor thesis, in relation to positive leadership in businesses?

As I am starting my Bachelor's thesis, I first need to think about the research question. Even if I have some ideas, I do not have yet the question itself, however here you can see the areas I...

18 October 2018 4,725 9 View

Is there additional essays to demonstrate RNA with RIN<7 is good to publish transcriptome analysis?

Recently I am engaged with the transcriptome analysis for differential expression of genes of a WT strain vs. its mutants (is a fungus). Mutant strains grow poorly; it is difficult to obtain good...

08 September 2018 1,774 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View